Countstar Mira FL – Fluorescence Cell Analyzer

The Countstar Mira FL is an automated fluorescence cell analyzer that integrates brightfield and fluorescence imaging with AI-driven analysis algorithms, delivering precise and versatile cell culture insights across a wide range of research applications.

Key Features

• 3-fold optical zoom (5.0x, 6.6x, and 8.0x) in brightfield and 5.0x in fluorescence, with patented Fixed Focus Technology enabling detection of cells and particles from 1 µm to 180 µm
• 5 MP CMOS color camera with dual fluorescence channels (Ex: 465–485 nm / Em: 535/40 nm and 600 nm LP) for simultaneous brightfield and fluorescence imaging
• Self-learning AI algorithms capable of analyzing complex image conditions including aggregated cells, irregular morphologies, and cell clusters
• Customizable BioApps — preset and expandable assay templates delivering results in seconds with minimal user input
• Proprietary 5-cavity chamber slides offering 250% higher sample capacity compared to classical 2-chamber chips, enabling up to five consecutive analyses
• 8-inch glove-sensitive HD touchscreen with an Android-based intuitive GUI for streamlined lab workflow
• 128 GB internal storage for up to 50,000 tests, with export options in CSV, PDF, and original image formats
• Compact and lightweight at 4 kg and 220 × 285 × 240 mm, designed to save valuable bench space

Applications

• AO/PI dual fluorescence viability — accurate cell density and viability analysis using the FRET-based AO/PI staining method, validated on CHO, HEK293, MCF-7, and Human T-cells; excludes akaryotes such as erythrocytes for cleaner results
• Trypan Blue viability assay — gold-standard cell concentration and viability measurement with additional data on cell diameter, aggregate formation, and morphology; validated on HEK293, MCF-7, CHO, and Human T-cells
• GFP/RFP transfection efficiency — quantification of fluorescent protein expression for viral vector and plasmid-based gene therapy workflows; validated on GFP-transfected HEK293 cells with results comparable to flow cytometry
• Apoptosis monitoring — early-stage detection of cell culture changes through cell shape factor analysis across mammalian cell cultures including CHO, MCF-7, and HEK293
• Algae and yeast object counting — concentration measurement and object counting for microbial samples including Chlorella species (green algae, 3–8 µm) and yeast cells
• Particle analysis — precise sizing and concentration measurement of cross-linked agarose particles (20–50 µm) and other non-biological objects
• Growth curve monitoring — real-time kinetic tracking of cell culture bioprocesses across multiple cultivations, applicable to mammalian, algae, and yeast cell cultures